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Binary vector pcambia-1300

WebpCAMBIA1300 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … http://www.biovector.net/product/1319.html

Frontiers Stable and Efficient Agrobacterium …

WebThe pCambia1301 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for … WebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … five \u0026 dive west edmonton mall https://redrockspd.com

pCAMBIA1300 Sequence and Map - SnapGene

WebPcambia 1300 (Search Vector Database) Vector type Plant Expression Selectable markers Hygromycin Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name FLAG-Pup (E) Promoter 35S Tag / Fusion Protein Triple Flag Tag … Webthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … WebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … five ugly babies part 4

A novel binary T-vector with the GFP reporter gene for promoter ...

Category:Schematic representation of pCAMBIA 1301. Binary vector …

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Binary vector pcambia-1300

pCambia1281Z Plant Expression Vector

WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,... WebA twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene.

Binary vector pcambia-1300

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WebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & … WebSep 1, 2015 · The pCambia 2300 vector assumes stable genomic insertion and expression in plant species ( Hajdukiewicz et al., 1994) and the proper Gateway®-like function and …

WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ... WebBinary vector pCAMBIA-1301 Taxonomy ID: 118389 (for references in articles please use NCBI:txid118389) current name. Binary vector pCAMBIA-1301. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid)

WebDec 18, 2008 · Vector Construction A transformation vector was constructed and denoted pCHO. It was derived from the Agrobacterium tumefaciens binary vector pCAMBIA 1300 ( http://www.cambia.org.au) by replacement of the original hph cassette driven by the 35S promoter from CaMV with newly constructed cassettes.

WebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for …

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents five \\u0026 divine - wernersvilleWebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … five ugly babiesWebBinary vector pCAMBIA-1300 NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, … can j1 be extendedWebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … can i とcan youの違いWebSep 21, 2024 · A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary vector pCambia-crTMV-CPPLRV. The significant levels of the chimeric virus enabled … can i zip tie my front license plateWebpCAMBIA2301 Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes. Sequence Author: Cambia Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspCAMBIA2301 Show … can i zip a powerpoint presentationWebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette. canizales dark gray area rug