WebJan 8, 2024 · PCR-amplified human TDP-43 and TDP-CTF were cloned into the pcDNA3.1 myc-BioID vector (Addgene) 12 as XhoI/KpnI fragments and cloned from GFP expression plasmids into the Kpn2I/MluI sites of the ... WebPbira Ha Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
BioID: A Screen for Protein-Protein Interactions - PMC
WebMay 5, 2016 · The status of BioID fusion proteins and biotinylated proteins is depicted on the left. Experimental procedures are depicted on the right. The NE proteins that have been analyzed by BioID are indicated with BioID fusion (red). Nup107-160 complex at the NPC is colored in red. (A) Generate expression BioID vector. (B) Stably express BioID fusion ... WebAug 13, 2024 · The same amino acid linker was used as in the CD28–PafA fusion. 12 million Jurkat cells were electroporated with 25 μg of DNA (10 μg of CD28–BirA*–Myc and 15 μg of empty vector for BioID ... small portable chicken coops
Addgene: MCS-BioID2-HA
WebOct 1, 2024 · Our BioID approach does not discard the possibility that the mt-PKA-BioID construct interacted with the identified interactors, such as TIM44-S80, outside of the mitochondrial matrix. ... Representative micrographs (n = 6) of HeLa cells expressing empty vector (pcDNA), PKA-Myc or mt-PKA-Myc and labelled with anti-Myc (green) and anti … Webmyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling. ... Expression vector of BirA-fused mouse … WebBioID control vectors: pDEST-pcDNA5-BirA-FLAG-GFP and pDEST-pcDNA5-FLAG-NLS-BirA. Vector controls for use with Invitrogen’s Flp-In T-REx system for BioID. Please refer to Couzens et al., 2013 in any publication using the BirA-FLAG-GFP vector and to Lambert et al, 2015 for the FLAG-NLS-BirA vector. Sequence. pDEST-pcDNA5-BirA-FLAG-GFP highlights man city real