WebSimilar kits are available from BioThema (Haninge, Sweden) ATP Kit SL (144-041). 2. Tris buffer with potassium acetate (Tris-KAc buffer): Bio-Orbit/BioThema kit buffer (100 mM Tris-acetate, 2 mM EDTA, pH 7.75 ± 0.05 at 25°C) augmented with 25 mM potassium acetate (see Subheading 1.2.3.). 2 M potassium acetate (1.963 g/10 mL water), WebCellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited.

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Web3. ATP release during cell lysis. 4. ATP release during platelet aggregation. Assay principles . Firefly luciferase catalyses the following reaction: luciferase . BioThema AB . ATP + D-luciferin + O. 2 . AMP+ PPi + oxyluciferin + CO. 2 + light The assay has been optimised to give a stable light at all ATP levels up to 10-6. mol/L. Instruments WebBioThema developes, produces and sells reagents and kits for measuring ATP by the firefly luciferase reaction. Applications include rapid cell counting, cell proliferation and … WebBioThema developes, produces and sells reagents and kits for measuring ATP by the firefly luciferase reaction. Applications include rapid cell counting, cell proliferation and cytotoxicity, immunoassays, monitoring enzymes and metabolites, reporter gene expression, hygiene monitoring, biomedicine and HTS in drug discovery. We sell directly to our customers … first horizon bank small business login