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WebLineage tracing K8-CreER T2;Pten Flox/Flox;R26-EYFP (K8PY) and control K8-CreER T2; ... Tamoxifen (#T5648, Sigma) was prepared as a 40 mg/mL solution in sunflower oil (#S5007, Sigma) with the addition of 96% ethanol (1:20 volume ratio). The solution was incubated at 55°C until the tamoxifen crystals were completely dissolved. Mice were … WebSep 22, 2015 · The Hoxa5 flox/flox; CMV-CreER T2+/ ... For oral administration of tamoxifen, a stock solution was prepared at 40 mg/ml, while three stock solutions were used for intragastric injection: one solution of 1 mg/ml for injection at P1, one solution of 1.5 mg/ml for injections at P2 and P3 and one solution of 2 mg/ml for injection at P4. β ... left on the scrap heap definition
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