Tīmeklis吸去上清，加入100μL新鲜DMEM高糖培养液，再加入配置好的10μL MTT溶液 (5mg/mL，0.5％MTT)，继续在恒温箱中培养4h。 吸去上清液后，每孔加入100μL的formazan溶解液，置摇床上低速振荡10min，在37℃条件下孵育3～4h。 待结晶物充分溶解后，在酶联免疫检测仪570nm处测量各个孔的吸光值。... Tīmeklis知乎，中文互联网高质量的问答社区和创作者聚集的原创内容平台，于 2011 年 1 月正式上线，以「让人们更好的分享知识、经验和见解，找到自己的解答」为品牌使命。知 …
MTT 溶液(5mg/ml)
http://www.basechem.org/chemical/16586 TīmeklisFormazan溶解液(Formazan Dissolving Solution)可以直接溶解培养液中的甲簪，无需离心吸除培养液，故可避免因操作中吸除了部分甲簪产物引起的操作误差，因此具有本 … goodfellow dod fire academy san angelo tx
甲䐶 - 維基百科，自由的百科全書
Tīmeklis2024. gada 1. marts · Europe PMC is an archive of life sciences journal literature. TīmeklisThe molecular cation MTT + is an important redox indicator that has been used in assays to study cytotoxicity, cell viability, and proliferation of living cells. This colorimetric assay method is based on the metabolic activity of viable cells. As a result, MTT is reduced to formazan (1- [4,5-dimethylthiazol-2-yl]-3,5-diphenylformazan). TīmeklisThe MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT, which is chemically 3-(4,5-di methyl thiazol-2-yl)-2,5-diphenyltetrazolium … goodfellow et al. 2016