Weba scatchard plot should give you a straight line with a slope that equals (-)affinity constant. look at these wikipedia pages: scatchard equation, scatchard plot. be happy you can use excel, i had to do them by hand (before excel, or visicalc, for that matter, existed). Thank you mdfenko, but I have a dimer that has different affinity to the ... Web1.特异性结合cd123的抗体,其特征在于,所述抗体包含有: 如seq id no.1‑3所示的或具有与其80%以上同一性的重链可变区的cdr1、cdr2和cdr3区域和如seq id no.10‑12所示的或具有与其80%以上同一性的轻链可变区的cdr1、cdr2和cdr3区域。 2.特异性结合cd123的抗原结合部分,其特征在于,所述抗原结合部分包含 ...
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WebScatchard analysis is a mode are linearizing your of a intensity binding experiment in order to determine bound constants. One creates a "secondary" plot of targeted binding/free radioligand concentration (Y axis) vs. specific binding (X axis). ... For info, consult to chapter inches Analyzing Data includes GraphPad Prism entitled "Analyzing ... WebThe current version of the program is 1.5.0.21 and was updated on 1/9/2006. It's available for users with the operating system Windows 95 and previous versions, and you can … rcw housing
The GraphPad Guide to Analyzing Radioligand …
WebThe pA 2 is a measure of the affinity of a competitive antagonist for its receptor. The determination of the pA 2 is made from experiments in which a fixed concentration of the antagonist is used along with graded concentrations of an agonist acting on the same receptor. The presence of the antagonist shifts the agonist dose—response curve to the … WebThis point dominates the linear regression calculations on the Scatchard graph. It has "pulled" the regression line to become shallower, resulting in an overestimate of the B … WebThis point dominates the linear regression calculations on the Scatchard graph. It has "pulled" the regression line to become shallower, resulting in an overestimate of the B max. Although it is inappropriate to analyze data by performing linear regression on a Scatchard plot, it is often helpful to display data as a Scatchard plot. Many people ... simultaneously detecting