Phellsgate 2

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August undefined, 2024

WebSep 28, 2010 · pHELLSGATE is an RNAi vector used to validate gene function in plants. In the present study, recombinant pHELLSGATE vector was constructed with amplicons of the gene serine threonine protein kinase (STPK) cloned from Piper colubrinum. WebPhellsgate 2 Vector Thermo Fisher Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Phellsgate 2 Vector, supplied by Thermo Fisher. Data for Phellsgate 2 …

A regulatory gene induces trichome formation and embryo ... - PNAS

WebDOI: 10.1111/j.1467-7652.2005.00149.x Abstract We describe here a vector system that allows dexamethasone-inducible RNAi against plant genes. The system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and … WebBriefly, the partial fragments of FaSAUR1 and FaSAUR2 (Supplementary Figure S5) were ligated into pHELLSGATE 2 vector (Shi et al., 2024) using BP Clonase (Invitrogen, MA, USA) to construct 35Spro... ibm inbox

Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing

WebpHELLSGATE 12 High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for … Web... original pHellsgate vector (Wesley et al. 2001) and pHellsgate 4 ( Fig. 1) were designed to facilitate a one-step cloning process where a single PCR product flanked by attB1 and … WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the … ibm inception

Analysis of the hairpin RNA targeting PnPR-like ... - ResearchGate

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WebJan 2, 2015 · Cinnamyl AlcoholDehydrogenase (CAD) is the final enzyme involved in the monolignol biosynthesispathway.Transgenic poplars were produced using RNAi strategy (pHellsgate 8 vector, Helliwell and Waterhouse, 2003) and exhibited the expectedphenotype (red xylem, reduced CAD activity). -RNAi-CAD transgenic poplars were produced using … WebWe have previously mapped Wo to an approximately 200-kb fragment by using an F 2 population from a cross between an introgression line 2–3 and woolly mutant LA3186 ... Suppression expressions of Wo and SlCycB2 were performed by using RNAi vector pHellsgate 2, which were ligated with the fragment of Wo and SlCycB2 (amplified with the … ibm in charlotteWebDec 23, 2001 · The pHELLSGATE vector was tested using 200 and 400 nt PCR products for two different genes. Many bacterial colonies were obtained on chloramphenicol … ibm in charlotte nc

"Web2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. pHELLSGATE 8 vector Sequence Copy Sequence " - Phellsgate 2

Phellsgate 2

Web豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ... WebpHELLSGATE, pWATERGATE, pSTARGATE and pOpOff contain Gateway TM recombination sites. Gateway TM is proprietary technology owned by Invitrogen and to the extent that CSIRO vectors incorporate Gateway TM technology, ... 5.2 ALL TERMS, CONDITIONS AND WARRANTIES (INCLUDING ANY WARRANTY AS TO MERCHANTABLE QUALITY OR …

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WebAfter inoculation with F. solani for 72 h, the PnPR-like expression level in the control leaves was about 5-times higher than that in the leaves transformed with pHellsgate 2-PnPR-like, which ... WebJul 24, 2024 · The optimization of the pHellsgate8-specific primer pairs 35S_S, OCS_S, and NPTII-3 centered on manipulating the following parameters: (i) final concentration of MgCl 2 in the PCR reaction, (ii) number of amplification cycles in the PCR program, and (iii) single versus multiplex PCR.

WebSep 19, 2016 · a, Total RNA extracted from the N. benthamiana plants expressing Hellsgate empty vector (EV), YFP or Bc-DCL1/2 RNAi constructs was examined by northern blot analysis to measure the expression... WebApr 5, 2024 · [0072] 实施例4：mswrky41基因转化紫花苜蓿的获得 [0073] 农杆菌准备：将在-80℃冰箱中保存的phb-mswrky41-flag和phellsgate-mswrky41rnai农杆菌菌株分别在含有抗生素为kan50+rif100和spe50+rif100的yeb固体培养基上划线活化，28℃培养48h后，挑取单克隆至含有相同抗生素的yeb液体培养 ...

WebAug 1, 2003 · The pHELLSGATE vectors A high-throughout cloning system to generate hpRNA constructs requires a vector, such as pHANNIBAL, to give directional insertion of a gene fragment into an inverted repeat conformation separated by an intron. WebJan 13, 2024 · The pHellsgate 2-PnPR-like recombinant plasmid and an empty pHellsgate two vector were inserted into separate A. tumefaciens EHA105 cells. Positive clones were …

WebJan 13, 2024 · sequence as well as the RNAi vector pHellsgate 2 were subjected to a BP recombination reaction using the BP Clonase TM II enzyme mix (Invitrogen, United States).

WebpHELLSGATE is an efficient, high-throughput and effective plant gene silencing vector. Vector Type. Gene Silencing Vector, Gateway Vector, Plant Dual Expression Vector ... Powder; Solution Size. 2 μg; 10 μL . High Copy/Low Copy. High Copy . Prokaryotic Resistance. Spectinomycin . Length. 18691 bp . Selectable Marker. ibm in businessWebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene fragments flanked by the appropriate att sites are recombined with the vector, an ihpRNA-encoding construct is produced ( Fig. 3 ). ibm in chester universityWebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in a single step from a single polymerase chain reaction product, while pHellsgate 8 requires a two-step process via an intermediate vector. ibm inc. armonk ny usaWeb4 beds, 3 baths, 2278 sq. ft. house located at 2302 Fieldgate Dr, Pittsburg, CA 94565. View sales history, tax history, home value estimates, and overhead views. APN ... ibm includeWebApr 8, 2024 · Zestimate® Home Value: $295,000. 902 Falgate Ct, Windy Hills, KY is a single family home that contains 2,200 sq ft and was built in 1979. It contains 4 bedrooms and 2 … ibm in chinaWebSep 19, 2016 · The plasmids pHellsgate-Bc-DCL1/2 and pHellsgate-Bc+Vd-DCLs were constructed following the methods outlined for the Hellsgate8.0 system 51. The Bc-DCL1/2 RNAi fragment was obtained by integrating Bc-DCL1 (252 bp) and Bc-DCL2 (238 bp) via overlapping PCR. mona weirichWebJan 1, 2005 · pHELLSGATE12 was created by inserting the catalase-I intron of castor bean as used in the p35SH-iH vector ( Wang et al., 1997) into the HindIII site of pHELLSGATE8. … mona wind life integrity