Phosphomotif antibodies

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August undefined, 2024

WebProtocols. As the epigenetics experts, Active Motif continues to develop new methods and helpful techniques to support your research. Our goal is to ensure your success with everything from gold standard assays to emerging approaches for epigenetics analysis. Check back often as we add new protocols, technotes and guides to this resource. WebSep 29, 2014 · Atg9A bears a conserved canonical 14-3-3ζ binding phosphomotif. (A) HA-Atg9A was overexpressed in HEK-293 cells, and cells were treated with hypoxia for the indicated times. Immunoprecipitated HA-Atg9A was immunoblotted with an anti-14-3-3ζ phosphomotif antibody (pMotif) and for endogenous 14-3-3ζ.

Identification of Phosphorylation Consensus Sequences and …

WebApr 12, 2024 · This antibody only detected protein when amino acids 166-575 were present, suggesting the antibody epitope resides within this region. The faint bands found in all lanes are likely the endogenous WAC protein isoforms expressed by the HEK293T cells. Overall, these data demonstrated that we could express the various WAC mutant proteins and ... WebProteins were extracted from each cell line and digested with trypsin. For phosphopeptide enrichment in serial immunoaffinity purifications, phosphomotif antibodies were used in the following order: PXpSP, pTP, RXRXXpS, and RXXpS where “X” represents any amino acid and “p” reflects the phosphorylation site. The samples were run in ... dethatching equipment for rent

Identification and Characterization of Neuronal Mitogen …

WebAntibodies—A key reagent for this study is the antibody for the Akt phosphomotif RXRXXpS/T, where X is any amino acid. This antibody, which is referred as the PAS antibody (phospho Akt substrate), was purchased from Cell Signaling Technology, Beverly, MA (catalog num-ber 9611). It is an affinity-purified rabbit antibody that has been shown WebOct 13, 2010 · Both studies 2,3 used phosphomotif-specific antibodies for immunoaffinity purification before MS analysis and quantified the effects of various pathway inhibitors on the newly identified Ser/Thr ... WebUnlike phosphoantibodies raised against a single amino acid sequence, phosphomotif antibodies (first developed by Cell Signaling Technology) recognize many different phosphoproteins, ... church adult day care

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Phosphomotif antibodies

Phosphoproteomic characterization of DNA damage response in …

WebPhosphoNET is an open-access, online resource developed by Kinexus Bioinformatics Corporation to foster the study of cell signalling systems to advance biomedical research in academia and industry. PhosphoNET is the worlds largest repository of known and predicted information on human phosphorylation sites, their evolutionary conservation, the ... WebThe antibody (termed "5557") recognized a variety of phosphoproteins in the brain, many of which were enriched in postsynaptic density fractions. The immunoblot pattern changed …

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Webantibodies, generally crosslinked to a resin, targeting a consensus phosphomotif or a specific phosphoresidue, such as phosphotyrosine (pTyr) 13,14. This last option is highly specific but is generally limited due to the cost of use. IAP can be less efficient in complex samples due to the high background WebMay 13, 2015 · The ability of anti-mHttexon1 antibodies and antibody fragments to reduce the aggregation and associated neurotoxicity has proven to be strongly dependent on the targeted epitope . Specifically targeting the Htt sequence between 1 and 17, which has been shown to play a key role in aggregation and HD pathology, seems to be an obvious choice.

WebFeb 11, 2013 · The scaffolding adapter protein Gab2 (Grb2-associated binder) participates in the signaling response evoked by various growth factors and cytokines. Gab2 is … WebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif …

Webever, phosphomotif antibodies have not been used so far for large scale proteomics identification of kinase substrates. To discover novel MAPK targets at the synapse, we raised a phosphospecific antibody against a peptide library repre-senting the MAPK consensus phosphorylation motif. Using this antibody we affinity-purified putative MAPK ... WebPhosphospecific antibodies developed against key drug-regulated phosphorylation sites provide an ideal tool for this approach because they can be used for …

WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies …

WebApr 1, 2009 · Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events.A, sequence of phosphopeptide library containing two consecutive consensus MAPK phosphorylation sites used for immunization to produce antibody 5557. church advance 2023WebNov 11, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. Upon treatment, melanoma cell lines responded by dramatically increasing phosphorylation on proteins containing a canonical DNA damage-response (DDR) motif, … church adult christmas party ideasWebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This … church adult christmas partyWebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … dethatching couch lawnWebPhosphoMotif Finder is especially useful for researchers who identify phosphorylation sites and would like to know if there is any specific enzyme associated with this function. The … church adult sunday school room furnitureWebphosphomotif antibodies, indicating the pos-sibility of direct ERK substrates. From this screen, 38 candidate targets were identi-fied by mass spectrometry (MS), includ-ing 13 new substrates that were verified by in vitro ERK phosphorylation. Further stud-ies of one of them, the nucleoporin Nup50, revealed an unexpected role of ERK signal- church advent bannersWebJan 1, 2005 · The antibody reactive with the phosphomotif generated by Akt, referred to here as the PAS (phospho Akt substrate) antibody, was purchased from Cell Signaling Technology, Beverly, MA (catalog number 9611). Rabbit antisera against peptides from the insulin-elicited phosphoproteins were generated for us by BioSource International, … dethatching for lawn mower