Shuming wang citrus gene editing

Author: glbw

August undefined, 2024

WebCRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be cre … WebAug 5, 2024 · We anticipate that future precision genome-editing efforts aimed at the CsACD2 promoter or coding sequences, via CRISPR technology (Jia et al., 2024; Wang 2024), to fine tune CsACD2 gene expression or interaction with SDE15 without causing spontaneous plant cell death could provide new possibilities to generate citrus resistance …

(PDF) Base Editors for Citrus Gene Editing - ResearchGate

WebNov 30, 2024 · Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming … dwtc hospitality

Citrus CsACD2 Is a Target of Candidatus Liberibacter Asiaticus in ...

WebMore recently, CRISPR/Cas9 mediated editing of citrus Nonexpressor of Pathogenesis-Related 3 (CsNPR3) gene, resulted in induced biotic stress tolerance in sweet orange cultivar 'N7-3 ′ (Mahmoud ... WebFeb 28, 2024 · citrus gene editing and have generated transgene-free gene-edited. citrus plants. ... Wang, N. (2024). The Citrus Huanglongbing Crisis and Potential Solutions. Mol. Plant 12, 607 ... WebJul 21, 2024 · In a companion study done by Wang, canker-resistant citrus plants have been generated by turning off a susceptibility gene showing the potential of this approach. Multiple genome-modified plants have been generated and are being tested for HLB resistance. In the process, the efficacy of genome editing has been increased. crystalloid bolus definition

Base Editors for Citrus Gene Editing Semantic Scholar

Targeted genome editing of sweet orange using Cas9/sgRNA.

WebOct 20, 2024 · The ABE base editor was successfully adapted to modify the TATA box in the promoter region of the canker susceptibility gene LOB1 from TATA to CACA in grapefruit and Hamlin sweet orange and has been used to generate transgene-free citrus via transient expression. Base editors, such as adenine base editors (ABE) and cytosine base editors … WebJan 28, 2014 · Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit and processed juice. We have sequenced the draft genome of a double-haploid sweet orange (C. sinensis cv. Valencia), … dwtc log inWebDec 9, 2016 · Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. dwt chicago

"WebWe propose to generate non-transgenic HLB resistant citrus varieties via genome editing of citrus susceptibility (S) genes to HLB using CRISPR ... Published: 2024 Citation: Huang X, Wang Y, Wang N. Base Editors for Citrus Gene Editing. Front Genome Ed. 2024 Feb 28;4:852867 ... Year Published: 2024 Citation: Shuming Wang, Hang ... " - Shuming wang citrus gene editing

Shuming wang citrus gene editing

Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the …

WebJia H, Wang N (2014b) Xcc-facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves. Plant Cell Rep 33:1993–2001 Jia H, Zhang Y, Orbovic V, Xu J, White FF, Jones JB, Wang N (2024) Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker. WebMar 18, 2024 · The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs (sgRNAs) to achieve multiplex gene editing in plant cells. One is to construct …

Did you know?

WebHigh concentrations of contaminants such as ammonia nitrogen and organic matter in full-strength wastewater severely inhibit the growth of microalgae, contributing to lower biomass accumulation and contaminant removal efficiency. To overcome this limitation, modified biochars prepared from pine sawdust and sugarcane bagasse were used in this study as … WebOct 24, 2024 · In a complementary approach, Nian Wang has been using a genome-editing technology, the CRISPR/Cas system, to delete and modify citrus DNA sequences in order to change the citrus proteins that are targeted by the bacterial effectors, ... the CRISPR/Cas system is being used to modify citrus genes targeted by CLas effectors, ...

WebSep 1, 2024 · Plant Cell Rep (2024) 36:1883–1887 DOI 10.1007/s00299-017-2202-4 FOCUS ARTICLE Rapid and efﬁcient CRISPR/Cas9 gene editing in Citrus using the YAO promoter 1 1 1,2 1 • • • Fei Zhang Chantal LeBlanc Vivian F. Irish Yannick Jacob Received: 3 July 2024 / Accepted: 23 August 2024 / Published online: 1 September 2024 Springer-Verlag GmbH … WebDisclosed herein are methods and materials for editing genes in citrus cells. Specifically exemplified is the implementation of an optimized Cas9 and the CRISPR type II class nuclease. Also exemplified is the use of a U6-1 promoter for driving expressing of editing constructs in citrus cells. Various protocols and sequences for editing genes are …

WebApr 7, 2014 · Given that Cas9/sgRNA works in transgenic citrus, Cas9/sgRNA-mediated citrus genome editing holds significant promise for studies of citrus gene function and for targeted genetic modification. In conclusion, Cas9/sgRNA appears to be a potentially valuable tool for creating new citrus cultivars with beneficial traits for both growers and … WebJan 5, 2024 · Abstract. CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in ...

WebOct 20, 2024 · Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. 126 . Nature Biotechnology. 36, 950-953. 127 . 128 . Figure legend . 129 . Figure 1. Highly efficient base editors for citrus gene editing. (a) Illustration of ABE8e adenine . 130 . base editing (ABE) system for citrus. CmYLCV, Cestrum yellow leaf curling virus ...

Webgenic Citrus plants were similar to those seen in pds mutants in other plant species (Meng et al. 2024), sug-gesting that the Arabidopsis YAO promoter is able to drive Cas9 expression at the level required for efﬁcient gene editing during early stages of shoot regeneration in Citrus. In some cases, phenotypically different shoots were crystalloid and colloidWebNov 2, 2015 · In Valencia sweet orange (Citrus sinensis) and Duncan grapefruit (Citrus paradisi Macf.), Cas9/sgRNA system was successfully employed to modify citrus CsPDS gene via Xcc-facilitated agroinfiltration, an optimized transient expression method in citrus (Jia and Wang, 2014a, b). dwtc meaningWebJun 22, 2024 · Genome editing offers promising solutions to genetic disorders by editing DNA sequences or modulating gene expression. The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) technology can be used to edit single or multiple genes in a wide variety of cell types and organisms in vitro and in … crystalloid examplesWebAug 1, 2024 · Engineering of citrus to obtain huanglongbing resistance. C Las cultures on hairy roots enable investigations in real C Las/citrus pathosystem. New C Las-resistant genotypes, sexually compatible with Citrus, have been reported. Putative target genes that may be involved in bacterial infection have been reported. dwtc online portalWebOct 20, 2024 · 107 First, we sought to improve the Cas9/CRISPR-mediated genome editing efficiency in citrus 108 using the phytoene desaturase (PDS) gene as a target. The PDS gene was chosen for editing 109 because of the obvious albino phenotype caused by homozygous or biallelic knockout mutation 110 of the PDS gene (Zhang et al., 2024a, … dwt collaborateWebCRISPR/Cas systems have been engineered for genome editing in many organisms, including plants. However, the gene editing efficiency in citrus via CRISPR technology remains too low to be implemented for genetic improvement in practice. Moreover, it is very difficult to obtain homozygous or biallelic knockout mutants in citrus. crystalloid definitionWebApr 7, 2014 · Given that Cas9/sgRNA works in transgenic citrus, Cas9/sgRNA-mediated citrus genome editing holds significant promise for studies of citrus gene function and for targeted genetic modification. In conclusion, Cas9/sgRNA appears to be a potentially valuable tool for creating new citrus cultivars with beneficial traits for both growers and … dwt cold cutting machine